Created by Dave Wood, Sheldon Moldoff, and Bob Kane, the character made his first appearance in Batman #121 (February 1959) as "Mr. Zero", a criminal scientist whose experimental "ice gun" backfires and spills cryogenic chemicals on him, forcing him to wear a sub-zero suit for survival and transforming him into a gimmicky stock villain who commits ice-themed crimes. The name "Mr. Freeze" was first used when the character was adapted for the 1960s Batman television series, in which he was played by three different actors: George Sanders, Otto Preminger and Eli Wallach. Mr. Freeze debuted in the episode "Instant Freeze" on February 2, 1966, and his comic book counterpart was renamed in Detective Comics #373 (March 1968). (via Wikipedia)
Nearly thirty years later, lead producers Paul Dini and Bruce Timm completely overhauled the character in Batman: The Animated Series. Artist Mike Mignola redesigned Mr. Freeze for the series at Timm's request, while Michael Ansara provided the character's voice. The episode "Heart of Ice", which was written by Dini and directed by Timm, aired on September 7, 1992, and retold Mr. Freeze's origin as Victor Fries, a scientist who turns to crime to find a cure for his cryogenically frozen, terminally ill wife, Nora. This revamped depiction of Mr. Freeze was critically acclaimed and led his characterization and backstory to be retconned into the mainstream comic book continuity.(via Wikipedia)