Lady Sio

Lady Sio is Jinno's younger sister appearing in Afro Samurai Resurrection. She asks Jinno to kill Afro, but Jinno refuses, calling Afro "his brother" of the sword and had a stronger bond. Unlike Otsuru, Sio and her brother are orphans who, with Afro, went to train with the Sword Master. Sio apparently lives with her wealthy and powerful foster family who had their own castle, and their replaced daughter, who treats Sio as a princess. Sio learns and dislikes that Afro killed her foster family though the words of her servant, Tomoe, imply that their lands were destroyed in the chaos that ensued after Afro takes the Number One headband from Justice, which means that her family's death is not Afro's fault. Rokutaro impales Sio and Jinno's damaged body with the broken sword.(via Wikipedia)